Intracerebral Infection with Dengue

Today's about intracerebral infection with dengue -3 virus induces meningoencephalitis and behavioral changes that precede lethality in mice.

Dengue, one of the most important arboviral disease of humans, can cause severe systemic disease. Although dengue virus (Denvir) was seen as a virus, not Neurotropik, dengue infection has recently been linked with a number of neurological syndromes, including encephalitis.

In this paper, the changes evaluatedbehavioral and inflammatory parameters in C57BL / 6 mice infected with non-adapted dengue virus 3 (Denvir-3) genotype I.

Methods: C57BL / 6 mice received 4x10 * 3 pfu of Denvir-3 by ICP. We evaluate the cerebral microvessels of trade leukocytes using intravital microscopy, and evaluated kemokin and the profile of cytokines by ELISA at 3 and 6 days after infection (pi).

Next, determine myeloperoxidase activity and Mar immunity of the population, and the histopathological analysis and immunostaining for the virus in brain tissue.

Results: All animals developed signs of encephalitis and death by day 8 pi motor behavior and parameters for the reduction of muscle strength in 7 days pi

We observed leukocyte rolling and adhesion in the cerebral microcirculation of mice infected with three six days pi Infection was followed by a significant increase of IFN-gamma, TNF-alpha, CCL2, CCL5, CXCL1 and CXCL2.

The histological examination showed evidence of reactive gliosis, and meningoencephalitis. Increasing the number of neutrophils, CD4 + and CD8 + T detected in the brains of infected animals, especially in day 6 pi

Tues immunoreactive to anti-NS-3 on the brain down.

Conclusion: Intracerebral infection with non-adapted Denvir-3 caused encephalitis and behavioral changes before death in mice.

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